Hotspot

trnG-GCC-trnfM-CAU

LSC · 39070-39285 · 216 bp

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Pi 0.00988
MarkerSeek score 64.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
216
variable_sites
4
pi
0.00988
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 265-294 bp 1.0 84.4
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 266-295 bp 1.0 84.4
trnG-GCC-trnfM-CAU_p3 ATGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.9 58.0 306-335 bp 1.0 83.9
trnG-GCC-trnfM-CAU_p4 AGAAGATGCGGGTTCGATTC GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 259-288 bp 1.0 83.8
trnG-GCC-trnfM-CAU_p5 GAGAAGATGCGGGTTCGATT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 260-289 bp 1.0 83.8