Hotspot

trnK-UUU-rps16

LSC · 4099-4814 · 716 bp

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Pi 0.0128
MarkerSeek score 68.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
716
variable_sites
20
pi
0.0128
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CTCAACCTACAGGAACGGTT 50.0 50.0 58.2 57.2 804-827 bp 1.0 76.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CTCAACCTACAGGAACGGTT 50.0 50.0 58.2 57.2 803-826 bp 1.0 76.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 813-836 bp 1.0 74.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAAAGGGCGCTCAACCTAC 50.0 52.6 58.2 58.1 812-835 bp 1.0 74.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTCAACCTACAGGAACGGTTT 50.0 47.6 58.2 57.9 803-826 bp 1.0 73.8