Hotspot

rps16

LSC · 5361-6514 · 1154 bp

Back to Turpinia

Pi 0.000867

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1154
variable_sites: 1
pi: 0.000867
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATACAATTCCCCCACACCCT	GTACCGACTGAACCGATGAC	50.0	55.0	58.3	58.1	1268-1269 bp	1.0	76.9
rps16_p2	ATACAATTCCCCCACACCCT	ACCGACTGAACCGATGACTA	50.0	50.0	58.3	58.2	1266-1267 bp	1.0	76.3
rps16_p3	ATACAATTCCCCCACACCCT	TACCGACTGAACCGATGACT	50.0	50.0	58.3	58.2	1267-1268 bp	1.0	76.3
rps16_p4	ATACAATTCCCCCACACCCT	TGTACCGACTGAACCGATGA	50.0	50.0	58.3	58.5	1269-1270 bp	1.0	74.0
rps16_p5	ATACAATTCCCCCACACCCT	ATGTACCGACTGAACCGATG	50.0	50.0	58.3	57.4	1270-1271 bp	1.0	73.0