Hotspot

petB

LSC · 80451-81889 · 1439 bp

Back to Turpinia

Pi 0.00139

MarkerSeek score 59.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1439
variable_sites: 2
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGTTCGACCGCGGAATTTAT	TCCTTCCCCAAGTCAAGTGA	45.0	50.0	57.7	58.2	1535-1537 bp	1.0	76.3
petB_p2	TAGTTTGGTAGTTCGACCGC	TCCTTCCCCAAGTCAAGTGA	50.0	50.0	57.6	58.2	1544-1546 bp	1.0	75.9
petB_p3	GTAGTTCGACCGCGGAATTT	TCCTTCCCCAAGTCAAGTGA	50.0	50.0	58.6	58.2	1537-1539 bp	1.0	73.5
petB_p4	AGTTCGACCGCGGAATTTAT	TGTTCCTTCCCCAAGTCAAG	45.0	50.0	57.7	57.3	1538-1540 bp	1.0	72.5
petB_p5	AGTTCGACCGCGGAATTTAT	TTCCTTCCCCAAGTCAAGTG	45.0	50.0	57.7	57.3	1536-1538 bp	1.0	72.5