Hotspot

matK

LSC · 2163-3683 · 1521 bp

Back to Turpinia

Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAGCCCTTACTCTTACGACA	TCCTCTACCCTCGGTTCAAA	47.6	50.0	58.2	57.7	1669 bp	1.0	68.0
matK_p2	CAGCCCTTACTCTTACGACA	TCCTCTACCCTCGGTTCAAA	50.0	50.0	56.7	57.7	1668 bp	1.0	67.0
matK_p3	TCAGCCCTTACTCTTACGACA	AATCCTCTACCCTCGGTTCA	47.6	50.0	58.2	57.5	1671 bp	1.0	66.1
matK_p4	TCAGCCCTTACTCTTACGACA	ATCCTCTACCCTCGGTTCAA	47.6	50.0	58.2	57.5	1670 bp	1.0	66.1
matK_p5	CAGCCCTTACTCTTACGACA	ATCCTCTACCCTCGGTTCAA	50.0	50.0	56.7	57.5	1669 bp	1.0	65.2