Hotspot

rpl32-trnL-UAG

SSC · 118552-119623 · 1072 bp

Back to Turpinia

Pi 0.000951

MarkerSeek score 61.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1072
variable_sites: 1
pi: 0.000951
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTATTGGGCAGCGTTGAAAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.7	58.0	1176-1195 bp	1.0	79.3
rpl32-trnL-UAG_p2	GTATTGGGCAGCGTTGAAAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.7	58.0	1177-1196 bp	1.0	79.2
rpl32-trnL-UAG_p3	GTATTGGGCAGCGTTGAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	1186-1205 bp	1.0	78.3
rpl32-trnL-UAG_p4	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	1170-1189 bp	1.0	77.9
rpl32-trnL-UAG_p5	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	1171-1190 bp	1.0	77.9