Hotspot

rbcL-accD

LSC · 61092-61866 · 775 bp

Back to Turpinia

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ATTCCGCCTTAGACAACACC	55.0	50.0	58.0	57.9	908 bp	1.0	78.9
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	CGCCTTAGACAACACCGAAT	55.0	50.0	58.0	58.3	904 bp	1.0	77.5
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	GGGCTTTCGCTTATACTGGT	55.0	50.0	58.0	57.7	984 bp	1.0	77.1
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	ATTCCGCCTTAGACAACACC	55.0	50.0	57.7	57.9	894 bp	1.0	76.9
rbcL-accD_p5	GGCTAGCAAATGGAGTCCTG	ATTCCGCCTTAGACAACACC	55.0	50.0	58.3	57.9	906 bp	1.0	76.6