Hotspot

ndhC-trnV-UAC

LSC · 55100-55855 · 756 bp

Back to Turpinia

Pi 0

MarkerSeek score 61.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ATCGGGACTAAAACTCCGGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	870-890 bp	1.0	79.2
ndhC-trnV-UAC_p2	TCGGGACTAAAACTCCGGAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	869-889 bp	1.0	77.4
ndhC-trnV-UAC_p3	CGGGACTAAAACTCCGGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	868-888 bp	1.0	76.6
ndhC-trnV-UAC_p4	ATCGGGACTAAAACTCCGGA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	869-889 bp	1.0	76.4
ndhC-trnV-UAC_p5	ATCGGGACTAAAACTCCGGA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.1	58.7	858-878 bp	1.0	75.3