Hotspot

trnF-GAA-ndhJ

LSC · 52676-53382 · 707 bp

Back to Turpinia

Pi 0

MarkerSeek score 61.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AATCATCCCCGACTGAAACG	55.0	50.0	58.0	58.0	869-885 bp	1.0	81.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AATCATCCCCGACTGAAACG	50.0	50.0	58.1	58.0	833-849 bp	1.0	81.5
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	AATCATCCCCGACTGAAACG	50.0	50.0	57.9	58.0	878-894 bp	1.0	81.1
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AATCATCCCCGACTGAAACG	55.0	50.0	57.9	58.0	857-873 bp	1.0	80.8
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TCCCCGACTGAAACGTATCT	55.0	50.0	58.0	58.2	864-880 bp	1.0	80.7