Hotspot

psaA-ycf3

LSC · 45471-46237 · 767 bp

Back to Turpinia

Pi 0.00522

MarkerSeek score 61.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 4
pi: 0.00522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCCGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	963-964 bp	1.0	79.3
psaA-ycf3_p2	TGAAAAATGACCCGGTCTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	945-946 bp	1.0	79.3
psaA-ycf3_p3	CTGGCCCATTCTTCGAAAGA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	947-948 bp	1.0	79.2
psaA-ycf3_p4	TCTGGCCCATTCTTCGAAAG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	948-949 bp	1.0	79.2
psaA-ycf3_p5	CTGGCCCATTCTTCGAAAGA	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	929-930 bp	1.0	79.2