Hotspot

trnC-GCA-petN

LSC · 29810-30762 · 953 bp

Back to Turpinia

Pi 0.00421

MarkerSeek score 61.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 4
pi: 0.00421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	CACTTCTTCCCCATACCACG	50.0	55.0	58.4	58.0	1080-1082 bp	1.0	77.4
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CCATACCACGAGTGAAAGGG	50.0	55.0	58.4	58.0	1070-1072 bp	1.0	77.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CCCATACCACGAGTGAAAGG	50.0	55.0	58.4	58.0	1071-1073 bp	1.0	77.4
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CCCCATACCACGAGTGAAAG	50.0	55.0	58.4	58.0	1072-1074 bp	1.0	77.4
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTCTTCCCCATACCACGAGT	50.0	50.0	58.4	58.1	1077-1079 bp	1.0	77.2