Hotspot

psbK-psbI

LSC · 8627-8950 · 324 bp

Back to Turpinia

Pi 0

MarkerSeek score 34.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCAAATTACCCGAGGCCTAC	TTCTTCACGCCCAGGATTAC	55.0	50.0	58.0	57.6	548 bp	1.0	76.5
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	462 bp	1.0	76.4
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	459 bp	1.0	74.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	460 bp	1.0	74.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	461 bp	1.0	74.4