Hotspot

rps16-trnQ-UUG

LSC · 6515-8022 · 1508 bp

Back to Turpinia

Pi 0.002

MarkerSeek score 60.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1508
variable_sites: 3
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATCAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1639-1652 bp	1.0	79.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1581-1594 bp	1.0	78.8
rps16-trnQ-UUG_p3	CCACATCGCTTCAAACGAAG	GAAATCAAAATGGGGCGTGG	50.0	50.0	57.8	58.0	1619-1632 bp	1.0	78.6
rps16-trnQ-UUG_p4	CCACATCGCTTCAAACGAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1561-1574 bp	1.0	78.1
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAAATCAAAATGGGGCGTGG	55.0	50.0	58.4	58.0	1653-1666 bp	1.0	77.5