Hotspot

trnK-UUU-rps16

LSC · 4443-5360 · 918 bp

Back to Turpinia

Pi 0.00109

MarkerSeek score 59.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 918
variable_sites: 1
pi: 0.00109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AACAGGCGCTCAATCTACAG	50.0	50.0	58.2	58.0	1043-1046 bp	1.0	78.8
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AACAGGCGCTCAATCTACAG	50.0	50.0	58.2	58.0	1042-1045 bp	1.0	78.8
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	TATTTCAACGAAGGCGGGAG	50.0	50.0	58.2	58.0	1010-1013 bp	1.0	78.8
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TATTTCAACGAAGGCGGGAG	50.0	50.0	58.2	58.0	1009-1012 bp	1.0	78.8
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TTGAAACAGGCGCTCAATCT	50.0	45.0	58.2	58.1	1046-1049 bp	1.0	78.1