primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU_p1 trnK-UUU 1 TGCGGATTTCTATACATATGATTTCG TGTTTTGATTGTGTCGTGAA 26 20 34.6 35.0 57.8 54.0 10.4 0.0 10.190547 1863 4442 2691-2697 1.000000 40.2 trnK-UUU_p2 trnK-UUU 2 TGCGGATTTCTATACATATGATTTCG TGTTTTGATTGTGTCGTGAAA 26 21 34.6 33.3 57.8 54.8 10.4 0.0 10.337251 1863 4442 2691-2697 1.000000 40.2 trnK-UUU_p3 trnK-UUU 3 GCGGATTTCTATACATATGATTTCGA TGTTTTGATTGTGTCGTGAA 26 20 34.6 35.0 57.6 54.0 10.4 0.0 10.413000 1863 4442 2690-2696 1.000000 40.2 trnK-UUU_p4 trnK-UUU 4 TGCGGATTTCTATACATATGATTTCG ATGTTTTGATTGTGTCGTGA 26 20 34.6 35.0 57.8 53.8 10.4 0.0 10.428480 1863 4442 2692-2698 1.000000 40.2 trnK-UUU_p5 trnK-UUU 5 AGAATGCGGATTTCTATACAT TGTTTTGATTGTGTCGTGAA 21 20 33.3 35.0 52.5 54.0 2.9 0.0 10.511319 1863 4442 2695-2701 1.000000 40.2 matK_p1 matK 1 TCAGCCCTTACTCTTACGACA TCCTCTACCCTCGGTTCAAA 21 20 47.6 50.0 58.2 57.7 0.0 0.0 1.515054 2163 3683 1669-1669 1.000000 68.0 matK_p2 matK 2 CAGCCCTTACTCTTACGACA TCCTCTACCCTCGGTTCAAA 20 20 50.0 50.0 56.7 57.7 0.0 0.0 1.633230 2163 3683 1668-1668 1.000000 67.0 matK_p3 matK 3 TCAGCCCTTACTCTTACGACA AATCCTCTACCCTCGGTTCA 21 20 47.6 50.0 58.2 57.5 0.0 0.0 1.745827 2163 3683 1671-1671 1.000000 66.1 matK_p4 matK 4 TCAGCCCTTACTCTTACGACA ATCCTCTACCCTCGGTTCAA 21 20 47.6 50.0 58.2 57.5 0.0 0.0 1.745827 2163 3683 1670-1670 1.000000 66.1 matK_p5 matK 5 CAGCCCTTACTCTTACGACA ATCCTCTACCCTCGGTTCAA 20 20 50.0 50.0 56.7 57.5 0.0 0.0 1.864003 2163 3683 1669-1669 1.000000 65.2 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 AAAGCCGAGTACTCTACCGT AACAGGCGCTCAATCTACAG 20 20 50.0 50.0 58.2 58.0 20.3 7.6 0.184156 4443 5360 1043-1046 1.000000 78.8 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 AAGCCGAGTACTCTACCGTT AACAGGCGCTCAATCTACAG 20 20 50.0 50.0 58.2 58.0 20.3 7.6 0.184156 4443 5360 1042-1045 1.000000 78.8 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 AAAGCCGAGTACTCTACCGT TATTTCAACGAAGGCGGGAG 20 20 50.0 50.0 58.2 58.0 20.3 0.0 0.186962 4443 5360 1010-1013 1.000000 78.8 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 AAGCCGAGTACTCTACCGTT TATTTCAACGAAGGCGGGAG 20 20 50.0 50.0 58.2 58.0 20.3 0.0 0.186962 4443 5360 1009-1012 1.000000 78.8 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 AAGCCGAGTACTCTACCGTT TTGAAACAGGCGCTCAATCT 20 20 50.0 45.0 58.2 58.1 20.3 7.6 0.272200 4443 5360 1046-1049 1.000000 78.1 rps16_p1 rps16 1 ATACAATTCCCCCACACCCT GTACCGACTGAACCGATGAC 20 20 50.0 55.0 58.3 58.1 0.0 0.0 0.400752 5361 6514 1268-1269 1.000000 76.9 rps16_p2 rps16 2 ATACAATTCCCCCACACCCT ACCGACTGAACCGATGACTA 20 20 50.0 50.0 58.3 58.2 0.0 0.0 0.475923 5361 6514 1266-1267 1.000000 76.3 rps16_p3 rps16 3 ATACAATTCCCCCACACCCT TACCGACTGAACCGATGACT 20 20 50.0 50.0 58.3 58.2 0.0 0.0 0.475923 5361 6514 1267-1268 1.000000 76.3 rps16_p4 rps16 4 ATACAATTCCCCCACACCCT TGTACCGACTGAACCGATGA 20 20 50.0 50.0 58.3 58.5 0.0 0.0 0.769262 5361 6514 1269-1270 1.000000 74.0 rps16_p5 rps16 5 ATACAATTCCCCCACACCCT ATGTACCGACTGAACCGATG 20 20 50.0 50.0 58.3 57.4 0.0 0.0 0.885408 5361 6514 1270-1271 1.000000 73.0 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AAGTCGCACGTTGCTTTCTA GAAATCAAAATGGGGCGTGG 20 20 45.0 50.0 58.1 58.0 8.1 0.0 0.148076 6515 8022 1639-1652 1.000000 79.4 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 6515 8022 1581-1594 1.000000 78.8 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 CCACATCGCTTCAAACGAAG GAAATCAAAATGGGGCGTGG 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.240991 6515 8022 1619-1632 1.000000 78.6 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 CCACATCGCTTCAAACGAAG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.8 58.1 0.0 16.9 0.308340 6515 8022 1561-1574 1.000000 78.1 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 GGATCGTGTCCTTCAAGTCG GAAATCAAAATGGGGCGTGG 20 20 55.0 50.0 58.4 58.0 2.9 0.0 0.378604 6515 8022 1653-1666 1.000000 77.5 psbK-psbI_p1 psbK-psbI 1 CCAAATTACCCGAGGCCTAC TTCTTCACGCCCAGGATTAC 20 20 55.0 50.0 58.0 57.6 4.4 0.0 0.433418 8627 8950 548-548 1.000000 76.5 psbK-psbI_p2 psbK-psbI 2 GTTTGGCAAGCTGCTGTAAG TTCTTCACGCCCAGGATTAC 20 20 50.0 50.0 57.9 57.6 1.4 0.0 0.452684 8627 8950 462-462 1.000000 76.4 psbK-psbI_p3 psbK-psbI 3 TGGCAAGCTGCTGTAAGTTT TTCTTCACGCCCAGGATTAC 20 20 45.0 50.0 58.3 57.6 1.4 0.0 0.705393 8627 8950 459-459 1.000000 74.4 psbK-psbI_p4 psbK-psbI 4 TTGGCAAGCTGCTGTAAGTT TTCTTCACGCCCAGGATTAC 20 20 45.0 50.0 58.3 57.6 1.4 0.0 0.705393 8627 8950 460-460 1.000000 74.4 psbK-psbI_p5 psbK-psbI 5 TTTGGCAAGCTGCTGTAAGT TTCTTCACGCCCAGGATTAC 20 20 45.0 50.0 58.3 57.6 1.4 0.0 0.705393 8627 8950 461-461 1.000000 74.4 psbI-trnS-GCU_p1 psbI-trnS-GCU 1 GTAATCCTGGGCGTGAAGAA TGGACTAAAGCGTCGGATTG 20 20 50.0 50.0 57.6 58.0 0.0 0.0 0.401172 9062 9263 294-298 1.000000 78.4 psbI-trnS-GCU_p2 psbI-trnS-GCU 2 GTAATCCTGGGCGTGAAGAA GTGGACTAAAGCGTCGGATT 20 20 50.0 50.0 57.6 58.0 0.0 0.0 0.402560 9062 9263 295-299 1.000000 78.4 psbI-trnS-GCU_p3 psbI-trnS-GCU 3 GTAATCCTGGGCGTGAAGAA ATTGGGAGAGATGGCTGAGT 20 20 50.0 50.0 57.6 58.1 0.0 0.0 0.506037 9062 9263 313-317 1.000000 77.5 psbI-trnS-GCU_p4 psbI-trnS-GCU 4 GTAATCCTGGGCGTGAAGAA GACTAAAGCGTCGGATTGCT 20 20 50.0 50.0 57.6 58.4 0.0 2.3 0.755013 9062 9263 292-296 1.000000 75.6 psbI-trnS-GCU_p5 psbI-trnS-GCU 5 GTAATCCTGGGCGTGAAGAA CTGAGTGGACTAAAGCGTCG 20 20 50.0 55.0 57.6 58.4 0.0 0.0 0.777729 9062 9263 299-303 1.000000 75.4 trnC-GCA-petN_p1 trnC-GCA-petN 1 GGGGGACTGCAAATCCTTTT CACTTCTTCCCCATACCACG 20 20 50.0 55.0 58.4 58.0 14.7 0.0 0.379667 29810 30762 1080-1082 1.000000 77.4 trnC-GCA-petN_p2 trnC-GCA-petN 2 GGGGGACTGCAAATCCTTTT CCATACCACGAGTGAAAGGG 20 20 50.0 55.0 58.4 58.0 14.7 4.6 0.379667 29810 30762 1070-1072 1.000000 77.4 trnC-GCA-petN_p3 trnC-GCA-petN 3 GGGGGACTGCAAATCCTTTT CCCATACCACGAGTGAAAGG 20 20 50.0 55.0 58.4 58.0 14.7 4.6 0.379667 29810 30762 1071-1073 1.000000 77.4 trnC-GCA-petN_p4 trnC-GCA-petN 4 GGGGGACTGCAAATCCTTTT CCCCATACCACGAGTGAAAG 20 20 50.0 55.0 58.4 58.0 14.7 4.6 0.379667 29810 30762 1072-1074 1.000000 77.4 trnC-GCA-petN_p5 trnC-GCA-petN 5 GGGGGACTGCAAATCCTTTT TTCTTCCCCATACCACGAGT 20 20 50.0 50.0 58.4 58.1 14.7 0.0 0.408506 29810 30762 1077-1079 1.000000 77.2 psaA-ycf3_p1 psaA-ycf3 1 TGAAAAATGACCCGGTCTGG ATCAAGCCGCTGAGTATTGG 20 20 50.0 50.0 58.1 58.0 18.5 0.0 0.136672 45471 46237 963-964 1.000000 79.3 psaA-ycf3_p2 psaA-ycf3 2 TGAAAAATGACCCGGTCTGG GGAAACAGGCTATAGCGCTT 20 20 50.0 50.0 58.1 58.0 18.5 18.7 0.140786 45471 46237 945-946 1.000000 79.3 psaA-ycf3_p3 psaA-ycf3 3 CTGGCCCATTCTTCGAAAGA ATCAAGCCGCTGAGTATTGG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.154819 45471 46237 947-948 1.000000 79.2 psaA-ycf3_p4 psaA-ycf3 4 TCTGGCCCATTCTTCGAAAG ATCAAGCCGCTGAGTATTGG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.154819 45471 46237 948-949 1.000000 79.2 psaA-ycf3_p5 psaA-ycf3 5 CTGGCCCATTCTTCGAAAGA GGAAACAGGCTATAGCGCTT 20 20 50.0 50.0 57.9 58.0 0.0 18.7 0.158933 45471 46237 929-930 1.000000 79.2 ycf3_p1 ycf3 1 TTGATTGATGGGGCCAACAA TGGGATCATTTTCTCACGAGAG 20 22 45.0 45.5 58.0 57.9 4.4 3.1 2.150187 46238 48207 2090-2090 1.000000 62.9 ycf3_p2 ycf3 2 TTTGATTGATGGGGCCAACA TGGGATCATTTTCTCACGAGAG 20 22 45.0 45.5 58.0 57.9 4.4 3.1 2.150187 46238 48207 2091-2091 1.000000 62.9 ycf3_p3 ycf3 3 TTGATTGATGGGGCCAACAA GGATCATTTTCTCACGAGAGGT 20 22 45.0 45.5 58.0 57.9 4.4 10.1 2.151548 46238 48207 2088-2088 1.000000 62.9 ycf3_p4 ycf3 4 TTTGATTGATGGGGCCAACA GGATCATTTTCTCACGAGAGGT 20 22 45.0 45.5 58.0 57.9 4.4 10.1 2.151548 46238 48207 2089-2089 1.000000 62.9 ycf3_p5 ycf3 5 TTGATTGATGGGGCCAACAA GTGGGATCATTTTCTCACGAGA 20 22 45.0 45.5 58.0 58.1 4.4 0.0 2.159421 46238 48207 2091-2091 1.000000 62.8 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 GGGATAGCTCAGCTGGTAGA AATCATCCCCGACTGAAACG 20 20 55.0 50.0 58.0 58.0 10.3 0.0 0.026681 52676 53382 869-885 1.000000 81.8 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 CCTCGTGTCACCAGTTCAAA AATCATCCCCGACTGAAACG 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.076071 52676 53382 833-849 1.000000 81.5 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 AGAATGGTCGGGATAGCTCA AATCATCCCCGACTGAAACG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.112280 52676 53382 878-894 1.000000 81.1 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 CTGGTAGAGCAGAGGACTGA AATCATCCCCGACTGAAACG 20 20 55.0 50.0 57.9 58.0 2.5 0.0 0.160474 52676 53382 857-873 1.000000 80.8 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 GGGATAGCTCAGCTGGTAGA TCCCCGACTGAAACGTATCT 20 20 55.0 50.0 58.0 58.2 10.3 0.0 0.159137 52676 53382 864-880 1.000000 80.7 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 ATCGGGACTAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.1 57.8 2.9 0.0 0.340654 55100 55855 870-890 1.000000 79.2 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TCGGGACTAAAACTCCGGAA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.4 57.8 5.2 0.0 0.565878 55100 55855 869-889 1.000000 77.4 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 CGGGACTAAAACTCCGGAAA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.5 57.8 5.2 0.0 0.658040 55100 55855 868-888 1.000000 76.6 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 ATCGGGACTAAAACTCCGGA GAAGGTCTACGGTTCGAGTC 20 20 50.0 55.0 58.1 57.5 2.9 0.0 0.684426 55100 55855 869-889 1.000000 76.4 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 ATCGGGACTAAAACTCCGGA GTTCGAGTCCGTATAGCCCT 20 20 50.0 55.0 58.1 58.7 2.9 0.0 0.826720 55100 55855 858-878 1.000000 75.3 rbcL-accD_p1 rbcL-accD 1 GAGGCTAGCAAATGGAGTCC ATTCCGCCTTAGACAACACC 20 20 55.0 50.0 58.0 57.9 18.2 0.0 0.149008 61092 61866 908-908 1.000000 78.9 rbcL-accD_p2 rbcL-accD 2 GAGGCTAGCAAATGGAGTCC CGCCTTAGACAACACCGAAT 20 20 55.0 50.0 58.0 58.3 18.2 0.0 0.317572 61092 61866 904-904 1.000000 77.5 rbcL-accD_p3 rbcL-accD 3 GAGGCTAGCAAATGGAGTCC GGGCTTTCGCTTATACTGGT 20 20 55.0 50.0 58.0 57.7 18.2 0.0 0.366783 61092 61866 984-984 1.000000 77.1 rbcL-accD_p4 rbcL-accD 4 GAGTCCTGAACTAGCTGCTG ATTCCGCCTTAGACAACACC 20 20 55.0 50.0 57.7 57.9 13.4 0.0 0.401528 61092 61866 894-894 1.000000 76.9 rbcL-accD_p5 rbcL-accD 5 GGCTAGCAAATGGAGTCCTG ATTCCGCCTTAGACAACACC 20 20 55.0 50.0 58.3 57.9 18.2 0.0 0.436256 61092 61866 906-906 1.000000 76.6 accD-psaI_p1 accD-psaI 1 AGTTATTTCAGCTCCACGCC CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.3 58.0 0.0 26.9 0.287937 63358 64052 821-826 1.000000 77.9 accD-psaI_p2 accD-psaI 2 GTTATTTCAGCTCCACGCCT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.3 58.0 0.0 26.9 0.287937 63358 64052 820-825 1.000000 77.9 accD-psaI_p3 accD-psaI 3 AGTTATTTCAGCTCCACGCC AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.3 57.9 0.0 26.8 0.364470 63358 64052 827-832 1.000000 77.3 accD-psaI_p4 accD-psaI 4 GTTATTTCAGCTCCACGCCT AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.3 57.9 0.0 26.8 0.364470 63358 64052 826-831 1.000000 77.3 accD-psaI_p5 accD-psaI 5 ATTTCAGCTCCACGCCTTTT CCATTGCAATTGCCGGAAAT 20 20 45.0 45.0 58.4 58.0 0.0 26.9 0.406181 63358 64052 817-822 1.000000 76.9 cemA_p1 cemA 1 AGATCGCATAGAGTCGACGA CCTGGATGGGTAGAAAGAGT 20 20 50.0 50.0 58.1 55.9 10.5 0.0 2.212296 66072 66767 835-835 1.000000 62.4 cemA_p2 cemA 2 CGCATAGAGTCGACGAATGA CCTGGATGGGTAGAAAGAGT 20 20 50.0 50.0 57.6 55.9 23.3 0.0 2.464361 66072 66767 831-831 1.000000 60.4 cemA_p3 cemA 3 TCGCATAGAGTCGACGAATG CCTGGATGGGTAGAAAGAGT 20 20 50.0 50.0 57.6 55.9 10.5 0.0 2.464361 66072 66767 832-832 1.000000 60.4 cemA_p4 cemA 4 GACGAATGAGGTGGGTTCAT CCTGGATGGGTAGAAAGAGT 20 20 50.0 50.0 57.6 55.9 0.0 0.0 2.482714 66072 66767 820-820 1.000000 60.2 cemA_p5 cemA 5 GATCGCATAGAGTCGACGAA CCTGGATGGGTAGAAAGAGT 20 20 50.0 50.0 57.3 55.9 10.5 0.0 2.744519 66072 66767 834-834 1.000000 58.1 clpP_p1 clpP 1 ATCGATCCTAACCGGACGAT ACGCTTCCACATCAAAGTGA 20 20 50.0 45.0 58.1 57.7 9.5 3.0 0.347556 75444 77550 2279-2281 1.000000 77.5 clpP_p2 clpP 2 ATCGATCCTAACCGGACGAT AACCCATTGTTACGCTTCCA 20 20 50.0 45.0 58.1 57.7 9.5 0.0 0.372945 75444 77550 2290-2292 1.000000 77.3 clpP_p3 clpP 3 ATCGATCCTAACCGGACGAT ACCCATTGTTACGCTTCCAC 20 20 50.0 50.0 58.1 58.5 9.5 0.0 0.562687 75444 77550 2289-2291 1.000000 75.7 clpP_p4 clpP 4 ATCGATCCTAACCGGACGAT CCCATTGTTACGCTTCCACA 20 20 50.0 50.0 58.1 58.5 9.5 0.0 0.563785 75444 77550 2288-2290 1.000000 75.7 clpP_p5 clpP 5 TCGATCCTAACCGGACGATT ACGCTTCCACATCAAAGTGA 20 20 50.0 45.0 58.3 57.7 9.5 3.0 0.570603 75444 77550 2278-2280 1.000000 75.7 petB_p1 petB 1 AGTTCGACCGCGGAATTTAT TCCTTCCCCAAGTCAAGTGA 20 20 45.0 50.0 57.7 58.2 35.9 0.0 0.490322 80451 81889 1535-1537 1.000000 76.3 petB_p2 petB 2 TAGTTTGGTAGTTCGACCGC TCCTTCCCCAAGTCAAGTGA 20 20 50.0 50.0 57.6 58.2 0.0 0.0 0.538074 80451 81889 1544-1546 1.000000 75.9 petB_p3 petB 3 GTAGTTCGACCGCGGAATTT TCCTTCCCCAAGTCAAGTGA 20 20 50.0 50.0 58.6 58.2 35.9 0.0 0.832495 80451 81889 1537-1539 1.000000 73.5 petB_p4 petB 4 AGTTCGACCGCGGAATTTAT TGTTCCTTCCCCAAGTCAAG 20 20 45.0 50.0 57.7 57.3 35.9 0.0 0.961698 80451 81889 1538-1540 1.000000 72.5 petB_p5 petB 5 AGTTCGACCGCGGAATTTAT TTCCTTCCCCAAGTCAAGTG 20 20 45.0 50.0 57.7 57.3 35.9 0.0 0.961698 80451 81889 1536-1538 1.000000 72.5 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 GTATTGGGCAGCGTTGAAAG AGCGTGTCTACCGATTTCAC 20 20 50.0 50.0 57.7 58.0 2.4 0.0 0.270402 118552 119623 1176-1195 1.000000 79.3 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 GTATTGGGCAGCGTTGAAAG CAGCGTGTCTACCGATTTCA 20 20 50.0 50.0 57.7 58.0 2.4 0.0 0.271773 118552 119623 1177-1196 1.000000 79.2 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 GTATTGGGCAGCGTTGAAAG TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 57.7 57.9 2.4 0.0 0.386849 118552 119623 1186-1205 1.000000 78.3 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 GGCAGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 58.4 58.0 14.0 0.0 0.437017 118552 119623 1170-1189 1.000000 77.9 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 GGCAGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 58.4 58.0 14.0 0.0 0.438388 118552 119623 1171-1190 1.000000 77.9