Hotspot

rpoC1

LSC · 21048-23833 · 2786 bp

Back to Tupistra

Pi 0.000359

MarkerSeek score 56.30

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2786
variable_sites: 1
pi: 0.000359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TTGGATAGACCCGTCTTCCC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.5	58.0	2960 bp	1.0	75.6
rpoC1_p2	TTGGATAGACCCGTCTTCCC	GCTCGTTCGAGAACTACGAT	55.0	50.0	58.5	57.5	2958 bp	1.0	72.3
rpoC1_p3	GGGCACCGATATCGTCTAAT	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.0	58.0	2938 bp	1.0	71.4
rpoC1_p4	GGGCACCGATATCGTCTAATC	TTGCTCGTTCGAGAACTACG	52.4	50.0	58.1	58.0	2938 bp	1.0	70.7
rpoC1_p5	GGATAGACCCGTCTTCCCA	TTGCTCGTTCGAGAACTACG	57.9	50.0	57.8	58.0	2958 bp	1.0	70.0