Hotspot

psbC

LSC · 34054-35475 · 1422 bp

Back to Tupistra

Pi 0.000703

MarkerSeek score 59.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCAGCGGAAGATCCTGAATT	ATAAAGGAGAATGGCTCGGC	50.0	50.0	58.3	57.7	1592 bp	1.0	75.3
psbC_p2	AGGTATTCGTGCTTGGATGG	ATAAAGGAGAATGGCTCGGC	50.0	50.0	57.7	57.7	1536 bp	1.0	75.3
psbC_p3	GCAGCGGAAGATCCTGAATT	AAAGGAGAATGGCTCGGCTA	50.0	50.0	58.3	58.8	1590 bp	1.0	71.1
psbC_p4	GCAGCGGAAGATCCTGAATT	TAAAGGAGAATGGCTCGGCT	50.0	50.0	58.3	58.8	1591 bp	1.0	71.1
psbC_p5	AGGTATTCGTGCTTGGATGG	AAAGGAGAATGGCTCGGCTA	50.0	50.0	57.7	58.8	1534 bp	1.0	71.0