Hotspot

clpP

LSC · 71471-73534 · 2064 bp

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Pi 0

MarkerSeek score 57.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2064
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGAATTCTGATTTGAAACGGA	AACTCATTCTTACGTTTCCA	36.4	35.0	56.4	52.5	2157 bp	1.0	40.0
clpP_p2	TCGAATTCTGATTTGAAACGGA	AAACTCATTCTTACGTTTCCA	36.4	33.3	56.4	53.4	2158 bp	1.0	40.0
clpP_p3	TCGAATTCTGATTTGAAACGGA	ACTCATTCTTACGTTTCCAT	36.4	35.0	56.4	52.2	2156 bp	1.0	40.0
clpP_p4	TCGAATTCTGATTTGAAACGGA	AACTCATTCTTACGTTTCCAT	36.4	33.3	56.4	53.2	2157 bp	1.0	40.0
clpP_p5	TCGAATTCTGATTTGAAACGGAA	AACTCATTCTTACGTTTCCA	34.8	35.0	57.1	52.5	2157 bp	1.0	40.0