Hotspot

psaJ-rpl33

LSC · 68616-69151 · 536 bp

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Pi 0.00187

MarkerSeek score 58.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 1
pi: 0.00187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATATCTTTCCACAGCGCCTG	AGAAATGCCTGGCGATTCTT	50.0	45.0	58.0	57.9	741 bp	1.0	78.7
psaJ-rpl33_p2	CATATCTTTCCACAGCGCCT	AGAAATGCCTGGCGATTCTT	50.0	45.0	58.0	57.9	742 bp	1.0	78.7
psaJ-rpl33_p3	TGGTTTGGATCTTTAGCGGG	AGAAATGCCTGGCGATTCTT	50.0	45.0	57.9	57.9	707 bp	1.0	78.1
psaJ-rpl33_p4	GGTTTGGATCTTTAGCGGGT	AGAAATGCCTGGCGATTCTT	50.0	45.0	57.9	57.9	706 bp	1.0	78.0
psaJ-rpl33_p5	ACATATCTTTCCACAGCGCC	AGAAATGCCTGGCGATTCTT	50.0	45.0	58.3	57.9	743 bp	1.0	76.4