Hotspot

psbE-petL

LSC · 66015-67300 · 1286 bp

Back to Tupistra

Pi 0.000778

MarkerSeek score 59.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 1
pi: 0.000778
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGGGCTGGGGTTATAGTTGA	50.0	50.0	57.9	57.7	1452 bp	1.0	77.4
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGGGCTGGGGTTATAGTTGA	50.0	50.0	57.9	57.7	1465 bp	1.0	77.1
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGGGCTGGGGTTATAGTTGA	45.0	50.0	58.2	57.7	1461 bp	1.0	76.4
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGGGCTGGGGTTATAGTTGA	50.0	50.0	57.8	57.7	1460 bp	1.0	76.3
psbE-petL_p5	CAACCCGCAATGAATAGGGA	CAATCAATAGGGCTGGGGTT	50.0	50.0	57.9	57.5	1460 bp	1.0	76.0