Hotspot

petA-psbJ

LSC · 64140-65245 · 1106 bp

Back to Tupistra

Pi 0.000904

MarkerSeek score 59.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 1
pi: 0.000904
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.1	58.0	1214 bp	1.0	79.1
petA-psbJ_p2	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.1	58.1	1215 bp	1.0	78.5
petA-psbJ_p3	CCCAGGGCCTTTTGTTTTTC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.7	58.0	1236 bp	1.0	77.7
petA-psbJ_p4	CCCAGGGCCTTTTGTTTTTC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.7	58.1	1237 bp	1.0	77.1
petA-psbJ_p5	TGGCATCCGTTATTTTGGCA	CCGGATTGGGTTCATCTCTG	45.0	55.0	58.4	58.0	1215 bp	1.0	76.1