Hotspot

psbK-psbI

LSC · 7416-7863 · 448 bp

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Pi 0

MarkerSeek score 55.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	TTACGTCCCGGATCGTTAGA	45.0	50.0	58.1	58.0	574-575 bp	1.0	79.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTACGTCCCGGATCGTTAGA	50.0	50.0	57.9	58.0	569-570 bp	1.0	79.4
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	ACGTCCCGGATCGTTAGATA	45.0	50.0	58.1	57.7	572-573 bp	1.0	77.6
psbK-psbI_p4	CTTTTGTTTGGCAAGCTGCT	TACGTCCCGGATCGTTAGAT	45.0	50.0	58.1	57.7	573-574 bp	1.0	77.6
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	ACGTCCCGGATCGTTAGATA	50.0	50.0	57.9	57.7	567-568 bp	1.0	77.6