Hotspot

rps15-ycf1

SSC · 121627-122046 · 420 bp

Back to Tulipa

Pi 0.0206
MarkerSeek score 59.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
420
variable_sites
68
pi
0.0206
unique_haplotype_count
49
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCGTCTTGCGGAATAACTGA TGTATCCACAATCCAGAATCAGT 45.0 39.1 57.0 57.3 473-501 bp 0.942529 51.9
rps15-ycf1_p2 TCGTCTTGCGGAATAACTGA TCCACAATCCAGAATCAGTT 45.0 40.0 57.0 54.2 469-497 bp 0.942529 51.4
rps15-ycf1_p3 TCTTCTTTTTCGTCTTGCGGA TGTATCCACAATCCAGAATCAGT 42.9 39.1 58.2 57.3 482-510 bp 0.931034 50.3
rps15-ycf1_p4 TCGTCTTGCGGAATAACTGAA TGTATCCACAATCCAGAATCAGT 42.9 39.1 57.7 57.3 473-501 bp 0.942529 49.7
rps15-ycf1_p5 TCTTCTTTTTCGTCTTGCGGA TCCACAATCCAGAATCAGTT 42.9 40.0 58.2 54.2 478-506 bp 0.931034 49.7