Hotspot

rpl32-trnL-UAG

SSC · 111673-112424 · 752 bp

Back to Tulipa

Pi 0.0256
MarkerSeek score 68.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
752
variable_sites
135
pi
0.0256
unique_haplotype_count
65
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAACAAACAAGTAATAGAATTTTGGA AGCAGCGTGTCTACCAATTT 26.9 45.0 55.2 57.8 788-843 bp 0.931034 48.7
rpl32-trnL-UAG_p2 CAACAAACAAGTAATAGAATTTTGGA CCTAAGAGCAGCGTGTCTAC 26.9 55.0 55.2 57.8 794-849 bp 0.931034 48.7
rpl32-trnL-UAG_p3 CAACAAACAAGTAATAGAATTTTGGA CTAAGAGCAGCGTGTCTACC 26.9 55.0 55.2 57.8 793-848 bp 0.931034 48.7
rpl32-trnL-UAG_p4 ACAAACAAGTAATAGAATTTTGGA TCCTAAGAGCAGCGTGTCTA 25.0 50.0 53.1 57.9 793-848 bp 0.931034 48.7
rpl32-trnL-UAG_p5 CAACAAACAAGTAATAGAATTTTGGA TCCTAAGAGCAGCGTGTCTA 26.9 50.0 55.2 57.9 795-850 bp 0.931034 48.6