Hotspot

ndhF-rpl32

SSC · 110794-111498 · 705 bp

Back to Tulipa

Pi 0.0151
MarkerSeek score 68.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
705
variable_sites
82
pi
0.0151
unique_haplotype_count
72
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAAGAAAGGGT TGCCGTTAAATACCCCTTCT 50.0 45.0 57.0 56.5 746-981 bp 0.931034 69.0
ndhF-rpl32_p2 CTGGAAGTGGAAGAAAGGGT TGCCGTTAAATACCCCTTCTT 50.0 42.9 57.0 57.3 746-981 bp 0.942529 67.6
ndhF-rpl32_p3 CTGGAAGTGGAAGAAAGGGT GCCGTTAAATACCCCTTCTTCT 50.0 45.5 57.0 58.1 745-980 bp 0.931034 64.0
ndhF-rpl32_p4 CTGGAAGTGGAAGAAAGGGT GCCGTTAAATACCCCTTCTTC 50.0 47.6 57.0 56.7 745-980 bp 0.942529 62.9
ndhF-rpl32_p5 CTGGAAGTGGAAGAAAGGGT TGCCGTTAAATACCCCTTCTTC 50.0 45.5 57.0 58.4 746-981 bp 0.942529 62.3