Hotspot

psaJ-rpl33

LSC · 65633-66245 · 613 bp

Back to Tulipa

Pi 0.0188
MarkerSeek score 64.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
613
variable_sites
99
pi
0.0188
unique_haplotype_count
68
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACCCGTGACATTTTGTACCC TTCTAGGGATCGATTCGGCT 50.0 50.0 58.1 58.0 1611-1967 bp 1.0 91.2
psaJ-rpl33_p2 CCCGTGACATTTTGTACCCA TTCTAGGGATCGATTCGGCT 50.0 50.0 58.1 58.0 1610-1966 bp 1.0 91.2
psaJ-rpl33_p3 ACAGGATTTGAACCCGTGAC TTCTAGGGATCGATTCGGCT 50.0 50.0 58.1 58.0 1622-1978 bp 1.0 91.1
psaJ-rpl33_p4 TTGGGTCTTTAGCGGGTCTA TTCTAGGGATCGATTCGGCT 50.0 50.0 58.1 58.0 1213-1415 bp 0.988506 91.0
psaJ-rpl33_p5 TTGGGTCTTTAGCGGGTCTA TGCACACATGTTCTGTTCGA 50.0 45.0 58.1 58.1 752-961 bp 0.988506 90.6