Hotspot

psbE-petL

LSC · 63140-64304 · 1165 bp

Back to Tulipa

Pi 0.0107
MarkerSeek score 65.60
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1165
variable_sites
128
pi
0.0107
unique_haplotype_count
71
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAAAAGAACGTTCTCCCGTG GCTGGGGTTATAGTTGAAGCA 50.0 47.6 57.3 58.0 750-1310 bp 0.988506 78.2
psbE-petL_p2 CAAAAGAACGTTCTCCCGTG TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.3 58.0 748-1308 bp 0.988506 78.2
psbE-petL_p3 CAGCAAAAGAACGTTCTCCC GCTGGGGTTATAGTTGAAGCA 50.0 47.6 57.3 58.0 753-1313 bp 0.988506 78.0
psbE-petL_p4 CAGCAAAAGAACGTTCTCCC TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.3 58.0 751-1311 bp 0.988506 78.0
psbE-petL_p5 CAAAAGAACGTTCTCCCGTG CCAATCAATAGAGCTGGGGT 50.0 50.0 57.3 57.0 762-1322 bp 0.954023 76.4