Hotspot

petA-psbJ

LSC · 61783-62360 · 578 bp

Pi 0.0215

MarkerSeek score 72.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 73
pi: 0.0215
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.7	58.1	734-877 bp	1.0	85.5
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	GCTGGTATTCCTGTGATCGG	55.0	55.0	58.8	58.1	745-888 bp	1.0	84.7
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.7	58.3	692-835 bp	1.0	84.0
petA-psbJ_p4	GATCCATTACGCGTCCAAGG	GGTTCATATTCCGGGTTGGG	55.0	55.0	58.8	58.3	703-846 bp	1.0	83.2
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.7	57.6	688-831 bp	1.0	83.1