Hotspot

ycf4-cemA

LSC · 59346-59892 · 547 bp

Pi 0.0152

MarkerSeek score 76.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 131
pi: 0.0152
unique_haplotype_count: 70
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAAAGCTGCCGAATTAGCCT	TGAGAGAGAGACCCACCAAG	45.0	55.0	58.2	58.1	558-708 bp	1.0	90.2
ycf4-cemA_p2	AAAAGCTGCCGAATTAGCCT	AGACCCACCAAGGCAAAAAT	45.0	45.0	58.2	57.9	550-700 bp	1.0	89.9
ycf4-cemA_p3	CCTTGACTGGCACTGATGAG	TGAGAGAGAGACCCACCAAG	55.0	55.0	58.3	58.1	604-754 bp	0.988506	88.9
ycf4-cemA_p4	CCTTGACTGGCACTGATGAG	AGACCCACCAAGGCAAAAAT	55.0	45.0	58.3	57.9	596-746 bp	0.988506	88.6
ycf4-cemA_p5	AAAAGCTGCCGAATTAGCCT	GGGAGGGAAGTCAATGCTTT	45.0	50.0	58.2	57.8	512-662 bp	0.988506	88.5