Hotspot

accD-psaI

LSC · 57579-58391 · 813 bp

Back to Tulipa

Pi 0.0131
MarkerSeek score 67.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
813
variable_sites
100
pi
0.0131
unique_haplotype_count
76
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TGAGTTATTTCAGCTCCACGG CCATTGCAATTGCCGGAAAT 47.6 45.0 58.0 58.0 874-984 bp 1.0 83.6
accD-psaI_p2 TGAGTTATTTCAGCTCCACGG AAGAAGCCATTGCAATTGCC 47.6 45.0 58.0 57.9 880-990 bp 1.0 83.0
accD-psaI_p3 GAGTTATTTCAGCTCCACGGT CCATTGCAATTGCCGGAAAT 47.6 45.0 58.0 58.0 873-983 bp 0.954023 81.7
accD-psaI_p4 GAGTTATTTCAGCTCCACGGT AAGAAGCCATTGCAATTGCC 47.6 45.0 58.0 57.9 879-989 bp 0.954023 81.1
accD-psaI_p5 AGTTATTTCAGCTCCACGGT CCATTGCAATTGCCGGAAAT 45.0 45.0 56.8 58.0 872-982 bp 0.954023 80.7