Hotspot

ycf3-trnS-GGA

LSC · 42954-43523 · 570 bp

Back to Tulipa

Pi 0.01
MarkerSeek score 64.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
570
variable_sites
58
pi
0.01
unique_haplotype_count
37
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTTTCCCCTGAAGTTGTCGG TTCCAATGCTACGCCTTGAA 50.0 45.0 58.0 57.8 665-728 bp 1.0 88.9
ycf3-trnS-GGA_p2 TTTTCCCCTGAAGTTGTCGG ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.0 58.2 715-778 bp 1.0 88.8
ycf3-trnS-GGA_p3 TTTTCCCCTGAAGTTGTCGG GGATTCGAACCCTCGGTAAG 50.0 55.0 58.0 57.8 703-766 bp 1.0 88.4
ycf3-trnS-GGA_p4 CCCCTGAAGTTGTCGGAATT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.8 58.2 711-774 bp 1.0 87.5
ycf3-trnS-GGA_p5 TTTTCCCCTGAAGTTGTCGG GGTAACGTTGGTGACTCGAT 50.0 50.0 58.0 57.6 739-802 bp 1.0 87.2