Hotspot

trnT-GGU-psbD

LSC · 30439-31584 · 1146 bp

Back to Tulipa

Pi 0.0102
MarkerSeek score 65.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1146
variable_sites
129
pi
0.0102
unique_haplotype_count
68
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CCCTCCGTAACCAATCATCC 55.0 55.0 58.1 57.7 1020-1376 bp 1.0 89.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CCCTCCGTAACCAATCATCC 50.0 55.0 58.1 57.7 1023-1379 bp 1.0 89.3
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA CCCTCCGTAACCAATCATCC 50.0 55.0 58.3 57.7 997-1353 bp 1.0 87.7
trnT-GGU-psbD_p4 GGCCCTTTTAACTCAGTGGT CCCTCCGTAACCAATCATCC 50.0 55.0 57.7 57.7 1035-1391 bp 1.0 87.6
trnT-GGU-psbD_p5 TGGTAGAGTAACGCCATGGT CCCTCCGTAACCAATCATCC 50.0 55.0 58.4 57.7 1019-1375 bp 1.0 86.5