Hotspot

trnE-UUC-trnT-GGU

LSC · 29431-30367 · 937 bp

Back to Tulipa

Pi 0.0219
MarkerSeek score 69.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
937
variable_sites
167
pi
0.0219
unique_haplotype_count
60
primer_available
yes
alignment_reliability
0.92
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 498-1041 bp 1.0 90.8
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 496-1039 bp 1.0 90.8
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 552-1095 bp 1.0 90.7
trnE-UUC-trnT-GGU_p4 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 477-1020 bp 1.0 90.3
trnE-UUC-trnT-GGU_p5 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 475-1018 bp 1.0 90.3