Hotspot

rpoB-trnC-GCA

LSC · 26280-27158 · 879 bp

Back to Tulipa

Pi 0.0107
MarkerSeek score 65.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
879
variable_sites
89
pi
0.0107
unique_haplotype_count
52
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG GCGACACCCAGATTTGAACT 50.0 50.0 58.2 58.5 1024-1108 bp 0.91954 82.4
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 999-1083 bp 0.91954 82.2
rpoB-trnC-GCA_p3 AATTCTTCCGTCAAGCCCTG CCTTACCACTTGGCCATGTC 50.0 55.0 58.2 58.5 978-1062 bp 0.896552 81.2
rpoB-trnC-GCA_p4 AATTCTTCCGTCAAGCCCTG TTTGTCAATCAGGCGACACC 50.0 50.0 58.2 58.8 1036-1120 bp 0.91954 80.2
rpoB-trnC-GCA_p5 AAATTCTTCCGTCAAGCCCT GCGACACCCAGATTTGAACT 45.0 50.0 57.4 58.5 1025-1109 bp 0.91954 79.2