Hotspot

trnS-GCU-trnG-GCC

LSC · 7871-8615 · 745 bp

Back to Tulipa

Pi 0.0144
MarkerSeek score 65.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
745
variable_sites
113
pi
0.0144
unique_haplotype_count
54
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 TGATGAGGGATAGCGGAGAG ACGAATCACACTTTTACCACT 55.0 38.1 58.1 55.5 676-1000 bp 1.0 63.0
trnS-GCU-trnG-GCC_p2 GAGGGATAGCGGAGAGAGAG ACGAATCACACTTTTACCACT 60.0 38.1 58.2 55.5 672-996 bp 1.0 62.3
trnS-GCU-trnG-GCC_p3 GATGAGGGATAGCGGAGAGA ACGAATCACACTTTTACCACT 55.0 38.1 57.8 55.5 675-999 bp 1.0 62.0
trnS-GCU-trnG-GCC_p4 ATGAGGGATAGCGGAGAGAG ACGAATCACACTTTTACCACT 55.0 38.1 57.8 55.5 674-998 bp 1.0 62.0
trnS-GCU-trnG-GCC_p5 TGATGAGGGATAGCGGAGAG GAACGAATCACACTTTTACCACT 55.0 39.1 58.1 57.3 678-1002 bp 1.0 62.0