Hotspot

trnK-UUU-rps16

LSC · 4117-4798 · 682 bp

Back to Tulipa

Pi 0.0113
MarkerSeek score 63.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
682
variable_sites
83
pi
0.0113
unique_haplotype_count
70
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGCCGAGTACTCTACCGTT ACATCTATCCCGATGAGCCA 50.0 50.0 58.2 58.0 913-960 bp 0.977011 89.3
trnK-UUU-rps16_p2 ACCTATACCCCGGAGACCTA TTCTCGAGCCGTATGAGGAT 55.0 50.0 58.2 58.0 1625-1672 bp 0.977011 87.5
trnK-UUU-rps16_p3 ACCTATACCCCGGAGACCTA ACATCTATCCCGATGAGCCA 55.0 50.0 58.2 58.0 1565-1612 bp 0.954023 86.2
trnK-UUU-rps16_p4 CTCGCGGAAAACCCTTTTTC TTCTCGAGCCGTATGAGGAT 50.0 50.0 58.0 58.0 1355-1402 bp 0.597701 72.8
trnK-UUU-rps16_p5 CTCGCGGAAAACCCTTTTTC ACATCTATCCCGATGAGCCA 50.0 50.0 58.0 58.0 1295-1342 bp 0.586207 72.3