Hotspot

ycf1

LSC · 94339-100542 · 6204 bp

Pi 0.00724

MarkerSeek score 49.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: LSC
length_bp: 6204
variable_sites: 100
pi: 0.00724
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGAGGAAGAACGGGAAGAA	TGGTTCGGAGACCACTACTT	50.0	50.0	58.0	58.0	563-611 bp	1.0	86.8
ycf1_p2	CCGGAAAAAGATGGAGGAGG	TGGTTCGGAGACCACTACTT	55.0	50.0	58.0	58.0	580-628 bp	1.0	86.6
ycf1_p3	GGGATTATGGAGTCTCCGGA	TGGTTCGGAGACCACTACTT	55.0	50.0	58.0	58.0	765-825 bp	1.0	86.4
ycf1_p4	GGGATTATGGAGTCTCCGGA	TTCTCTGCGACGTTTTGGAA	55.0	45.0	58.0	58.1	972-1032 bp	1.0	85.6
ycf1_p5	TCGTGAATAGTCCTCGCTCT	TGGTTCGGAGACCACTACTT	50.0	50.0	58.0	58.0	908-968 bp	0.8	77.7