Hotspot

accD

SSC · 37663-38919 · 1257 bp

Back to Tsuga

Pi 0.012
MarkerSeek score 60.60
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
SSC
length_bp
1257
variable_sites
32
pi
0.012
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 AAGCCAACAAAATTTTCCCT CCCGAGTTCAACAATGAGGA 35.0 50.0 54.1 57.5 1352-1418 bp 1.0 48.2
accD_p2 AAGCCAACAAAATTTTCCCT TCCCGAGTTCAACAATGAGG 35.0 50.0 54.1 57.5 1353-1419 bp 1.0 48.2
accD_p3 AGCCAACAAAATTTTCCCTT CCCGAGTTCAACAATGAGGA 35.0 50.0 54.1 57.5 1351-1417 bp 1.0 48.2
accD_p4 AGCCAACAAAATTTTCCCTT TCCCGAGTTCAACAATGAGG 35.0 50.0 54.1 57.5 1352-1418 bp 1.0 48.2
accD_p5 AAGCCAACAAAATTTTCCCTT CCCGAGTTCAACAATGAGGA 33.3 50.0 55.0 57.5 1352-1418 bp 1.0 47.2