Hotspot

rps7-trnL-CAA

LSC · 110959-112725 · 1767 bp

Back to Tsuga

Pi 0.00905
MarkerSeek score 62.20
Primer available yes
Label rps7-trnL-CAA

Feature Metadata

label_name
rps7-trnL-CAA
feature_type
igs
region
LSC
length_bp
1767
variable_sites
36
pi
0.00905
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-trnL-CAA_p1 GCAGAGGCCAATAGAGCTTT GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 1764-1878 bp 1.0 85.1
rps7-trnL-CAA_p2 GCAGAGGCCAATAGAGCTTT CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 1762-1876 bp 1.0 85.1
rps7-trnL-CAA_p3 GCAGAGGCCAATAGAGCTTT GCGTGTCTACCATTTCACCA 50.0 50.0 58.0 58.2 1759-1873 bp 1.0 83.6
rps7-trnL-CAA_p4 GCAGAGGCCAATAGAGCTTT TCTCGCGTGTCTACCATTTC 50.0 50.0 58.0 57.7 1763-1877 bp 1.0 83.0
rps7-trnL-CAA_p5 GCAGAGGCCAATAGAGCTTT TCGCGTGTCTACCATTTCAC 50.0 50.0 58.0 58.3 1761-1875 bp 1.0 82.8