Hotspot

rpl32-trnV-GAC

LSC · 107251-108301 · 1051 bp

Back to Tsuga

Pi 0.00681
MarkerSeek score 61.50
Primer available yes
Label rpl32-trnV-GAC

Feature Metadata

label_name
rpl32-trnV-GAC
feature_type
igs
region
LSC
length_bp
1051
variable_sites
16
pi
0.00681
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnV-GAC_p1 GTACGCTTTTTCGGAACTGC TTGACGTGGTGGAAGTCATC 50.0 50.0 58.1 57.8 1016-1116 bp 1.0 83.4
rpl32-trnV-GAC_p2 GTACGCTTTTTCGGAACTGC CGTGGTGGAAGTCATCAGTT 50.0 50.0 58.1 57.8 1012-1112 bp 1.0 83.4
rpl32-trnV-GAC_p3 GTACGCTTTTTCGGAACTGC CTTGACGTGGTGGAAGTCAT 50.0 50.0 58.1 57.8 1017-1117 bp 1.0 83.4
rpl32-trnV-GAC_p4 AAAAGCTTTTATTGCGGCCC TTGACGTGGTGGAAGTCATC 45.0 50.0 57.9 57.8 1089-1189 bp 1.0 83.0
rpl32-trnV-GAC_p5 AAAAGCTTTTATTGCGGCCC CGTGGTGGAAGTCATCAGTT 45.0 50.0 57.9 57.8 1085-1185 bp 1.0 83.0