Hotspot

trnF-GAA-trnL-UAA

LSC · 66361-66746 · 386 bp

Back to Tsuga

Pi 0.0152
MarkerSeek score 59.10
Primer available yes
Label trnF-GAA-trnL-UAA

Feature Metadata

label_name
trnF-GAA-trnL-UAA
feature_type
igs
region
LSC
length_bp
386
variable_sites
13
pi
0.0152
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-trnL-UAA_p1 TGGCACGAGGATTTTCAGTC CCGTTGGCTTTATAGACCGT 50.0 50.0 58.2 57.7 377-480 bp 1.0 82.3
trnF-GAA-trnL-UAA_p2 TGGCACGAGGATTTTCAGTC TAGGAGGAAAATCCGTTGGC 50.0 50.0 58.2 57.6 389-492 bp 1.0 81.4
trnF-GAA-trnL-UAA_p3 TGGCACGAGGATTTTCAGTC GGAGGAAAATCCGTTGGCTT 50.0 50.0 58.2 58.5 387-490 bp 1.0 81.1
trnF-GAA-trnL-UAA_p4 CCAGATTTGAACTGGTGGCA CCGTTGGCTTTATAGACCGT 50.0 50.0 58.4 57.7 392-495 bp 1.0 80.8
trnF-GAA-trnL-UAA_p5 GTGGCACGAGGATTTTCAGT CCGTTGGCTTTATAGACCGT 50.0 50.0 58.5 57.7 378-481 bp 1.0 80.1