Hotspot

petA-psbJ

SSC · 43212-43930 · 719 bp

Back to Tsuga

Pi 0.00779
MarkerSeek score 68.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
SSC
length_bp
719
variable_sites
13
pi
0.00779
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGTTCAATTGGCCGAGATGA TCGTGATTGGTCTACTGGGT 45.0 50.0 57.2 58.1 793-813 bp 1.0 77.5
petA-psbJ_p2 AGTTCAATTGGCCGAGATGA TGGAAGGATCCCTCTTTGGT 45.0 50.0 57.2 57.9 837-857 bp 1.0 77.4
petA-psbJ_p3 AGTTCAATTGGCCGAGATGA TACCACTGGAAGGATCCCTC 45.0 55.0 57.2 57.8 843-863 bp 1.0 76.4
petA-psbJ_p4 AAGTTCAATTGGCCGAGATGA TGGAAGGATCCCTCTTTGGT 42.9 50.0 57.9 57.9 838-858 bp 1.0 75.1
petA-psbJ_p5 AGTTCAATTGGCCGAGATGAA TGGAAGGATCCCTCTTTGGT 42.9 50.0 57.9 57.9 837-857 bp 1.0 75.1