Hotspot

rbcL-accD

SSC · 37037-37662 · 626 bp

Back to Tsuga

Pi 0.0125
MarkerSeek score 65.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
SSC
length_bp
626
variable_sites
18
pi
0.0125
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGTCCTGAACTAGCTGCTG CTACGCTTGTCTTCGAACCA 55.0 50.0 57.7 57.9 737-777 bp 1.0 82.4
rbcL-accD_p2 GAGTCCTGAACTAGCTGCTG TACGCTTGTCTTCGAACCAC 55.0 50.0 57.7 58.2 736-776 bp 1.0 81.1
rbcL-accD_p3 GCTGCTGCTTGTGAAGTATG CTACGCTTGTCTTCGAACCA 50.0 50.0 57.5 57.9 724-764 bp 1.0 80.5
rbcL-accD_p4 GAGTCCTGAACTAGCTGCTG TCTACGCTTGTCTTCGAACC 55.0 50.0 57.7 57.7 738-778 bp 1.0 80.1
rbcL-accD_p5 GCTGCTGCTTGTGAAGTATG TACGCTTGTCTTCGAACCAC 50.0 50.0 57.5 58.2 723-763 bp 1.0 79.2