Hotspot

trnT-GGU-trnH-GUG

SSC · 29887-30009 · 123 bp

Back to Tsuga

Pi 0.0195
MarkerSeek score 62.90
Primer available yes
Label trnT-GGU-trnH-GUG

Feature Metadata

label_name
trnT-GGU-trnH-GUG
feature_type
igs
region
SSC
length_bp
123
variable_sites
6
pi
0.0195
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnH-GUG_p1 GCCCTTTTAACTCAGCGGTA CCAAGGCAGTGGATTGTGAA 50.0 50.0 57.9 58.4 249-251 bp 1.0 78.4
trnT-GGU-trnH-GUG_p2 GGGAGGCCCTTTTAACTCAG CCAAGGCAGTGGATTGTGAA 55.0 50.0 57.9 58.4 254-256 bp 1.0 78.1
trnT-GGU-trnH-GUG_p3 GCCCTTTTAACTCAGCGGTA AGTTGTTCGGTTGGTTTCCA 50.0 45.0 57.9 57.9 290-291 bp 0.8 71.5
trnT-GGU-trnH-GUG_p4 GGGAGGCCCTTTTAACTCAG AGTTGTTCGGTTGGTTTCCA 55.0 45.0 57.9 57.9 295-296 bp 0.8 71.3
trnT-GGU-trnH-GUG_p5 GGCGTAAGTCATCGGTTCAA AGTTGTTCGGTTGGTTTCCA 50.0 45.0 58.3 57.9 253-254 bp 0.8 70.3