Hotspot

psbM-trnD-GUC

SSC · 27592-28529 · 938 bp

Back to Tsuga

Pi 0.007
MarkerSeek score 62.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
SSC
length_bp
938
variable_sites
14
pi
0.007
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCAAGCATTCTTTTTGGCGT TTAGAGTACCGCCCTGTCAA 40.0 50.0 56.8 58.1 1008-1094 bp 1.0 75.1
psbM-trnD-GUC_p2 ACTGCTTGCAGTGCTTCAA TTAGAGTACCGCCCTGTCAA 47.4 50.0 58.2 58.1 1023-1109 bp 1.0 74.8
psbM-trnD-GUC_p3 TCAAGCATTCTTTTTGGCGT ATTGGTTAGAGTACCGCCCT 40.0 50.0 56.8 58.1 1013-1099 bp 1.0 74.6
psbM-trnD-GUC_p4 ACTGCTTGCAGTGCTTCAA ATTGGTTAGAGTACCGCCCT 47.4 50.0 58.2 58.1 1028-1114 bp 1.0 74.4
psbM-trnD-GUC_p5 ACTGCTTGCAGTGCTTCAAG TTAGAGTACCGCCCTGTCAA 50.0 50.0 59.3 58.1 1023-1109 bp 1.0 73.8