Hotspot

petN-psbM

SSC · 26752-27495 · 744 bp

Back to Tsuga

Pi 0.00633
MarkerSeek score 65.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
SSC
length_bp
744
variable_sites
11
pi
0.00633
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.93
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CGGTATAACTTGGGCTGCTT ACGCCAAAAAGAATGCTTGA 50.0 40.0 58.0 56.8 794-843 bp 1.0 75.4
petN-psbM_p2 GGGGGAGAAGTGGGCTATAA ACGCCAAAAAGAATGCTTGA 55.0 40.0 58.2 56.8 735-784 bp 1.0 74.3
petN-psbM_p3 CGGTATAACTTGGGCTGCTT CCCTTACGCCAAAAAGAATGC 50.0 47.6 58.0 58.4 799-848 bp 1.0 73.6
petN-psbM_p4 CGGTATAACTTGGGCTGCTT TCGTTGGATTTCCTACTGCT 50.0 45.0 58.0 56.6 830-879 bp 1.0 73.2
petN-psbM_p5 ACTTGGGCTGCTTTAATGGT ACGCCAAAAAGAATGCTTGA 45.0 40.0 57.7 56.8 787-836 bp 1.0 73.2