Hotspot

psbI-trnS-GCU

IRb · 7865-7939 · 75 bp

Back to Tsuga

Pi 0.0347
MarkerSeek score 66.50
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
IRb
length_bp
75
variable_sites
5
pi
0.0347
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CTATCGAACGATCCAGGACG GGGTTCGAATCCCTCTCTCT 55.0 55.0 57.7 58.2 131-140 bp 1.0 82.6
psbI-trnS-GCU_p2 ATCGAACGATCCAGGACGTA GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.3 58.2 129-138 bp 1.0 82.6
psbI-trnS-GCU_p3 CTATCGAACGATCCAGGACG TTTTGGAGAGATGGCTGAGC 55.0 50.0 57.7 57.9 200-209 bp 1.0 81.9
psbI-trnS-GCU_p4 ATCGAACGATCCAGGACGTA TTTTGGAGAGATGGCTGAGC 50.0 50.0 58.3 57.9 198-207 bp 1.0 81.9
psbI-trnS-GCU_p5 TATCGAACGATCCAGGACGT TTTTGGAGAGATGGCTGAGC 50.0 50.0 58.3 57.9 199-208 bp 1.0 81.9