Hotspot

rpl16

LSC · 85645-87347 · 1703 bp

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Pi 0.0157

MarkerSeek score 59.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1703
variable_sites: 24
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGAGCAATCGCACCAAAAAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2275-2442 bp	1.0	84.8
rpl16_p2	TCTCAATTCCCGAGCAATCG	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.1	58.0	2285-2452 bp	1.0	84.5
rpl16_p3	TATCATGCCGTTGTCACGTT	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.9	58.0	2192-2359 bp	1.0	84.3
rpl16_p4	TTATCATGCCGTTGTCACGT	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.9	58.0	2193-2360 bp	1.0	84.3
rpl16_p5	CTGTCTCAATTCCCGAGCAA	GCAGGGTTCCCTTACAAACA	50.0	50.0	57.9	58.0	2288-2455 bp	1.0	84.2