Hotspot

psbA

LSC · 92-1153 · 1062 bp

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Pi 0.00188

MarkerSeek score 46.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCTTGTATCTTGGGGGATTGC	GTGCTTGGGAGTCCTTGAAA	47.6	50.0	57.7	58.0	1168 bp	0.5	49.5
psbA_p2	CTTGTATCTTGGGGGATTGCT	GTGCTTGGGAGTCCTTGAAA	47.6	50.0	57.7	58.0	1167 bp	0.5	49.4
psbA_p3	TCTTGTATCTTGGGGGATTGC	CATAGGGAAAGTCGTGTGCA	47.6	50.0	57.7	57.9	1425 bp	0.5	48.9
psbA_p4	TCTTGTATCTTGGGGGATTGC	AATACGTGTGCTTGGGAGTC	47.6	50.0	57.7	57.9	1175 bp	0.5	48.9
psbA_p5	TCTTGTATCTTGGGGGATTGC	ACATAGGGAAAGTCGTGTGC	47.6	50.0	57.7	57.9	1426 bp	0.5	48.9