Hotspot

clpP

LSC · 73372-75551 · 2180 bp

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Pi 0.0069

MarkerSeek score 47.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2180
variable_sites: 15
pi: 0.0069
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATCTGAACTGAACGAGTCGC	CAATTGGCTGATCCGATCGA	50.0	50.0	58.0	58.1	2826-2915 bp	1.0	82.2
clpP_p2	ACTGAACGAGTCGCACATAC	CAATTGGCTGATCCGATCGA	50.0	50.0	58.0	58.1	2820-2909 bp	1.0	82.2
clpP_p3	CTGAACGAGTCGCACATACA	CAATTGGCTGATCCGATCGA	50.0	50.0	58.0	58.1	2819-2908 bp	1.0	82.2
clpP_p4	GTTGGCATGGCGTGTCTATA	CAATTGGCTGATCCGATCGA	50.0	50.0	58.1	58.1	2698-2787 bp	1.0	82.1
clpP_p5	AGTCGCACATACACCCTAGT	CAATTGGCTGATCCGATCGA	50.0	50.0	58.2	58.1	2812-2901 bp	1.0	81.1